Active membrane transport and receptor proteins from bacteria
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چکیده
منابع مشابه
Active membrane transport and receptor proteins from bacteria.
A general strategy for the expression of bacterial membrane transport and receptor genes in Escherichia coli is described. Expression is amplified so that the encoded proteins comprise 5-35% of E. coli inner membrane protein. Depending upon their topology, proteins are produced with RGSH6 or a Strep tag at the C-terminus. These enable purification in mg quantities for crystallization and NMR st...
متن کاملBinding proteins and membrane transport.
The recent studies have clearly established two types of active transport systems. One type is membrane-bound and can be observed in membrane vesicles and the other type is osmotic-shock-sensitive and requires binding proteins to produce active transport. It appears that the membrane-bound systems derive cellular energy from the energy-rich membrane state which can be formed from respiration or...
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Energy-generating sugar efJlux during the steady state The net uptake of a non-metabolized glucose analogue is linear for a few minutes and then slowly starts to level off to a plateau value because the influx of sugar is balanced by sugar efflux which is mainly catalysed by the sugar transport system. The rate of sugar exchange is very high and can be twice that of the net uptake rate. Despite...
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Active transport of proline was observed in both electron transport particles and cell membrane ghosts from Mycobacterium phlei. The transport of proline was dependent upon substrate oxidation and the presence of Li+ or Na+, but not upon the formation of a high energy phosphorylated intermediate. The requirement for Na+ was different from Na+ coupled transport systems which have been described;...
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Active transport of proline was observed in both electron transport particles and cell membrane ghosts from Mycobacterium phlei. The transport of proline was dependent upon substrate oxidation and the presence of Li+ or Na+, but not upon the formation of a high energy phosphorylated intermediate. The requirement for Na+ was different from Na+ coupled transport systems which have been described;...
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ژورنال
عنوان ژورنال: Biochemical Society Transactions
سال: 2005
ISSN: 0300-5127,1470-8752
DOI: 10.1042/bst0330867